The little brown myotis is a small bat with long, glossy fur that ranges in color from pale tan to dark chocolate brown. It is found in wooded areas throughout most of Canada and the northern half of the U.S., with the exception of desert or arid areas.
There are also a few isolated populations farther south. In summer, females often form nursery colonies in buildings. Nursery colonies have also been found in tree hollows, rock crevices and bridges. These bats are also common residents of bat houses. In winter, little brown bats hibernate in caves and mines.